Out Of This World Info About How To Detect Protein Protein Interactions
Of course this requires that you have a good antibody against.
How to detect protein protein interactions. Pife has mainly been used in single molecule studies to detect protein association with dna or rna. Pcas can be used to detect ppi between proteins of any molecular weight and expressed at their endogenous levels. Look at frequently asked questions for preparing and processing samples for cytogenetics analysis, including copy number and somatic mutations in oncology samples.
First i use glutathine resin to test, three systems, a + b, b + c and a + b + c, in the same time, mix a with the resin, mix c with the resin, use pbs to wash the resin, then add pbs to disperse. Here we applied pife for steady state quantification of protein/na. In far wb, proteins in a cell lysate containing prey.